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p jnk  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc p jnk
    Knocking down SKA2-induced cell-cycle arrest and apoptosis through the SKA2/ROS/ATM axis in GC cell lines (A) Western blotting analysis of γ-H2AX (Ser139), ATM, p -ATM (Ser1981), p -Chk2 (Thr68), and SKA2 expression in SNU638 and NUGC3 SKA2-knockdown cell lines. (B) Western blotting analysis of SKA2 overexpression in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against γ-H2AX (Ser139), ATM, p -ATM (Ser1981), p -Chk2 (Thr68), and SKA2. (C) Western blotting analysis of KU-55933 treatment in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against cyclin D1, cyclin A2, cyclin B1, p -Chk2 (Thr68), PARP, <t>Cleaved-Caspase3,</t> <t>JNK,</t> p -JNK (Thr183/Tyr185), ATM, p -ATM (Ser1981), and SKA2. (D) Western blotting analysis of BML-277 treatment in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against cyclin D1, cyclin A2, cyclin B1, p -Chk2 (Thr68), and SKA2. (E) Western blotting analysis of P38, p-P38 (Thr180/Tyr182), ERK, p -ERK1/2 (Thr202/Tyr204), JNK, p -JNK (Thr183/Tyr185), and SKA2 expression in SNU638 and NUGC3 SKA2-knockdown cell lines. (F) Western blotting analysis of the rescue effect of SKA2 overexpression on MAPK pathway markers (ERK, p -ERK1/2, JNK, and p -JNK) in SNU638 and NUGC3 SKA2-knockdown cell lines. (G) Western blotting analysis of JNK-IN-8 treatment in SNU638 SKA2-knockdown cell lines using antibodies against PARP, cleaved-caspase3, JNK, p -JNK (Thr183/Tyr185), and SKA2. α-Tubulin was used as the internal control for all blots. Representative blotting images are shown from 3 independent experiments.
    P Jnk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+jnk/pmc12990383-16-0-2
    Average 86 stars, based on 1 article reviews
    p jnk - by Bioz Stars, 2026-09
    86/100 stars

    Images

    1) Product Images from "SKA2 promotes gastric cancer progression by regulating glutathione metabolism"

    Article Title: SKA2 promotes gastric cancer progression by regulating glutathione metabolism

    Journal: iScience

    doi: 10.1016/j.isci.2026.115202

    Knocking down SKA2-induced cell-cycle arrest and apoptosis through the SKA2/ROS/ATM axis in GC cell lines (A) Western blotting analysis of γ-H2AX (Ser139), ATM, p -ATM (Ser1981), p -Chk2 (Thr68), and SKA2 expression in SNU638 and NUGC3 SKA2-knockdown cell lines. (B) Western blotting analysis of SKA2 overexpression in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against γ-H2AX (Ser139), ATM, p -ATM (Ser1981), p -Chk2 (Thr68), and SKA2. (C) Western blotting analysis of KU-55933 treatment in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against cyclin D1, cyclin A2, cyclin B1, p -Chk2 (Thr68), PARP, Cleaved-Caspase3, JNK, p -JNK (Thr183/Tyr185), ATM, p -ATM (Ser1981), and SKA2. (D) Western blotting analysis of BML-277 treatment in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against cyclin D1, cyclin A2, cyclin B1, p -Chk2 (Thr68), and SKA2. (E) Western blotting analysis of P38, p-P38 (Thr180/Tyr182), ERK, p -ERK1/2 (Thr202/Tyr204), JNK, p -JNK (Thr183/Tyr185), and SKA2 expression in SNU638 and NUGC3 SKA2-knockdown cell lines. (F) Western blotting analysis of the rescue effect of SKA2 overexpression on MAPK pathway markers (ERK, p -ERK1/2, JNK, and p -JNK) in SNU638 and NUGC3 SKA2-knockdown cell lines. (G) Western blotting analysis of JNK-IN-8 treatment in SNU638 SKA2-knockdown cell lines using antibodies against PARP, cleaved-caspase3, JNK, p -JNK (Thr183/Tyr185), and SKA2. α-Tubulin was used as the internal control for all blots. Representative blotting images are shown from 3 independent experiments.
    Figure Legend Snippet: Knocking down SKA2-induced cell-cycle arrest and apoptosis through the SKA2/ROS/ATM axis in GC cell lines (A) Western blotting analysis of γ-H2AX (Ser139), ATM, p -ATM (Ser1981), p -Chk2 (Thr68), and SKA2 expression in SNU638 and NUGC3 SKA2-knockdown cell lines. (B) Western blotting analysis of SKA2 overexpression in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against γ-H2AX (Ser139), ATM, p -ATM (Ser1981), p -Chk2 (Thr68), and SKA2. (C) Western blotting analysis of KU-55933 treatment in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against cyclin D1, cyclin A2, cyclin B1, p -Chk2 (Thr68), PARP, Cleaved-Caspase3, JNK, p -JNK (Thr183/Tyr185), ATM, p -ATM (Ser1981), and SKA2. (D) Western blotting analysis of BML-277 treatment in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against cyclin D1, cyclin A2, cyclin B1, p -Chk2 (Thr68), and SKA2. (E) Western blotting analysis of P38, p-P38 (Thr180/Tyr182), ERK, p -ERK1/2 (Thr202/Tyr204), JNK, p -JNK (Thr183/Tyr185), and SKA2 expression in SNU638 and NUGC3 SKA2-knockdown cell lines. (F) Western blotting analysis of the rescue effect of SKA2 overexpression on MAPK pathway markers (ERK, p -ERK1/2, JNK, and p -JNK) in SNU638 and NUGC3 SKA2-knockdown cell lines. (G) Western blotting analysis of JNK-IN-8 treatment in SNU638 SKA2-knockdown cell lines using antibodies against PARP, cleaved-caspase3, JNK, p -JNK (Thr183/Tyr185), and SKA2. α-Tubulin was used as the internal control for all blots. Representative blotting images are shown from 3 independent experiments.

    Techniques Used: Western Blot, Expressing, Knockdown, Over Expression, Control

    Related Articles

    other:

    Article Title: SKA2 promotes gastric cancer progression by regulating glutathione metabolism
    Article Snippet: p-JNK , Cell Signaling Technology , Cat#9255; RRID: AB_2307321.

    Western Blot:

    Article Title: USP25 regulates atherosclerosis by restricting RIPK1-mediated inflammatory responses.
    Article Snippet: TNF-α (Cat#: 10291-TA) was purchased from R&D Systems (Minnesota, USA). .. Antibodies against RIPK1 (Cat#: 3493, 1: 1000, RRID: AB_2305314), p-RIPK1 (Cat#: 31122, 1: 1000, RRID: AB_2799000), p-IKKα/β (Cat#: 2697, 1: 1000, RRID: AB_2079382), IKKα (Cat#: 2682, 1:1000, RRID: AB_331626), p-p65 (Cat#: 3033, 1: 1000 for Western bolt and 1: 200 for immunofluorescence, RRID: AB_331284), p65 (Cat#: 8242, 1: 1000, RRID: AB_10859369), p-p38 (Cat#: 4511, 1: 1000, RRID: AB_2139682), p38 (Cat#: 8690, 1: 1000, RRID: AB_10999090), p-JNK (Cat#: 4668S, 1:1000, RRID: AB_ 823588), JNK (Cat#: 9252S, 1:1000, RRID: AB_2250373), p-ERK1/2 (Cat#: 4370S, 1:1000, RRID: AB_2315112), ERK1/2 (Cat#: 4695S, 1:1000, RRID: AB_ 390779), GAPDH (Cat#: 5174, 1: 1000, RRID: AB_ 10622025), α-SMA (Cat#: 19245, 1: 200 for immunofluorescence, RRID: AB_2734735), and Ubiquitin (Cat#: 3936, 1: 1000, RRID: AB_331292) were purchased from Cell Signaling Technology (Danvers, MA, USA). .. Antibodies against FLAG (Cat#: 20543-1-AP, 1: 1000, RRID: AB_11232216), MYC (Cat#: 60003-2-Ig, 1: 1000, RRID: AB_2734122), HA (Cat#: 51064-2-AP, 1: 1000, RRID: AB_11042321), CD36 (Cat#: 18836-1-AP, 1:1000, RRID: AB_10597244), ASK1 (Cat#: 28201-1-AP, 1: 1000, RRID: AB_2782957), MLKL (Cat#: 66675-1-Ig, 1:1000, RRID: AB_2882029), and p-ASK1 (Cat#: 28846- 1-AP, 1: 1000, RRID: AB_2881220) were purchased from Proteintech (Wuhan, China).

    Article Title: Geniposide ameliorates ferroptosis through the ROS/p38 MAPK/NCOA4/GPX4-mediated ferritinophagy in a co-culture system of HMC3 and SH-SY5Y cells.
    Article Snippet: .. The antibodies used for immunoblotting were as follows: LC3B, p62, Atg7, p-p38 MAPK, total p38 MAPK, p-ERK, total ERK, p-JNK, total JNK, HO-1, p-NFκB, total NF-κB, Iba-1, SQSTM1/p62, LC3B, Atg5, Beclin-1, (Cell Signaling Technology, Danvers, MA); TNF-α, IL-1β (Santa Cruz Biotechnology Inc., Santa Cruz, TX); β-actin (Sigma-Aldrich Inc., St. Louis, MO); COX-2, BDNF, LAMP2, NCOA4, GPX4, FTH1, Transferrin, xCT (Abcam, Waltham, MA). .. Afterward, membranes were incubated with the secondary antibody for 60 min. Immunoreactive proteins were visualized using a chemiluminescent substrate (Amersham Life Sciences Inc., Arlington Heights, IL).

    Article Title: USP25 regulates atherosclerosis by restricting RIPK1-mediated inflammatory responses
    Article Snippet: TNF-α (Cat#: 10291-TA) was purchased from R&D Systems (Minnesota, USA). .. Antibodies against RIPK1 (Cat#: 3493, 1: 1000, RRID: AB_2305314 ), p-RIPK1 (Cat#: 31122, 1: 1000, RRID: AB_2799000 ), p-IKKα/β (Cat#: 2697, 1: 1000, RRID: AB_2079382 ), IKKα (Cat#: 2682, 1:1000, RRID: AB_331626 ), p-p65 (Cat#: 3033, 1: 1000 for Western bolt and 1: 200 for immunofluorescence, RRID: AB_331284 ), p65 (Cat#: 8242, 1: 1000, RRID: AB_10859369 ), p-p38 (Cat#: 4511, 1: 1000, RRID: AB_2139682 ), p38 (Cat#: 8690, 1: 1000, RRID: AB_10999090 ), p-JNK (Cat#: 4668S, 1:1000, RRID: AB_823588 ), JNK (Cat#: 9252S, 1:1000, RRID: AB_2250373 ), p-ERK1/2 (Cat#: 4370S, 1:1000, RRID: AB_2315112 ), ERK1/2 (Cat#: 4695S, 1:1000, RRID: AB_390779 ), GAPDH (Cat#: 5174, 1: 1000, RRID: AB_10622025 ), α-SMA (Cat#: 19245, 1: 200 for immunofluorescence, RRID: AB_2734735 ), and Ubiquitin (Cat#: 3936, 1: 1000, RRID: AB_331292 ) were purchased from Cell Signaling Technology (Danvers, MA, USA). .. Antibodies against FLAG (Cat#: 20543-1-AP, 1: 1000, RRID: AB_11232216 ), MYC (Cat#: 60003-2-Ig, 1: 1000, RRID: AB_2734122 ), HA (Cat#: 51064-2-AP, 1: 1000, RRID: AB_11042321 ), CD36 (Cat#: 18836-1-AP, 1:1000, RRID: AB_10597244 ), ASK1 (Cat#: 28201-1-AP, 1: 1000, RRID: AB_2782957 ), MLKL (Cat#: 66675-1-Ig, 1:1000, RRID: AB_2882029 ), and p-ASK1 (Cat#: 28846-1-AP, 1: 1000, RRID: AB_2881220 ) were purchased from Proteintech (Wuhan, China).

    Immunofluorescence:

    Article Title: USP25 regulates atherosclerosis by restricting RIPK1-mediated inflammatory responses.
    Article Snippet: TNF-α (Cat#: 10291-TA) was purchased from R&D Systems (Minnesota, USA). .. Antibodies against RIPK1 (Cat#: 3493, 1: 1000, RRID: AB_2305314), p-RIPK1 (Cat#: 31122, 1: 1000, RRID: AB_2799000), p-IKKα/β (Cat#: 2697, 1: 1000, RRID: AB_2079382), IKKα (Cat#: 2682, 1:1000, RRID: AB_331626), p-p65 (Cat#: 3033, 1: 1000 for Western bolt and 1: 200 for immunofluorescence, RRID: AB_331284), p65 (Cat#: 8242, 1: 1000, RRID: AB_10859369), p-p38 (Cat#: 4511, 1: 1000, RRID: AB_2139682), p38 (Cat#: 8690, 1: 1000, RRID: AB_10999090), p-JNK (Cat#: 4668S, 1:1000, RRID: AB_ 823588), JNK (Cat#: 9252S, 1:1000, RRID: AB_2250373), p-ERK1/2 (Cat#: 4370S, 1:1000, RRID: AB_2315112), ERK1/2 (Cat#: 4695S, 1:1000, RRID: AB_ 390779), GAPDH (Cat#: 5174, 1: 1000, RRID: AB_ 10622025), α-SMA (Cat#: 19245, 1: 200 for immunofluorescence, RRID: AB_2734735), and Ubiquitin (Cat#: 3936, 1: 1000, RRID: AB_331292) were purchased from Cell Signaling Technology (Danvers, MA, USA). .. Antibodies against FLAG (Cat#: 20543-1-AP, 1: 1000, RRID: AB_11232216), MYC (Cat#: 60003-2-Ig, 1: 1000, RRID: AB_2734122), HA (Cat#: 51064-2-AP, 1: 1000, RRID: AB_11042321), CD36 (Cat#: 18836-1-AP, 1:1000, RRID: AB_10597244), ASK1 (Cat#: 28201-1-AP, 1: 1000, RRID: AB_2782957), MLKL (Cat#: 66675-1-Ig, 1:1000, RRID: AB_2882029), and p-ASK1 (Cat#: 28846- 1-AP, 1: 1000, RRID: AB_2881220) were purchased from Proteintech (Wuhan, China).

    Article Title: USP25 regulates atherosclerosis by restricting RIPK1-mediated inflammatory responses
    Article Snippet: TNF-α (Cat#: 10291-TA) was purchased from R&D Systems (Minnesota, USA). .. Antibodies against RIPK1 (Cat#: 3493, 1: 1000, RRID: AB_2305314 ), p-RIPK1 (Cat#: 31122, 1: 1000, RRID: AB_2799000 ), p-IKKα/β (Cat#: 2697, 1: 1000, RRID: AB_2079382 ), IKKα (Cat#: 2682, 1:1000, RRID: AB_331626 ), p-p65 (Cat#: 3033, 1: 1000 for Western bolt and 1: 200 for immunofluorescence, RRID: AB_331284 ), p65 (Cat#: 8242, 1: 1000, RRID: AB_10859369 ), p-p38 (Cat#: 4511, 1: 1000, RRID: AB_2139682 ), p38 (Cat#: 8690, 1: 1000, RRID: AB_10999090 ), p-JNK (Cat#: 4668S, 1:1000, RRID: AB_823588 ), JNK (Cat#: 9252S, 1:1000, RRID: AB_2250373 ), p-ERK1/2 (Cat#: 4370S, 1:1000, RRID: AB_2315112 ), ERK1/2 (Cat#: 4695S, 1:1000, RRID: AB_390779 ), GAPDH (Cat#: 5174, 1: 1000, RRID: AB_10622025 ), α-SMA (Cat#: 19245, 1: 200 for immunofluorescence, RRID: AB_2734735 ), and Ubiquitin (Cat#: 3936, 1: 1000, RRID: AB_331292 ) were purchased from Cell Signaling Technology (Danvers, MA, USA). .. Antibodies against FLAG (Cat#: 20543-1-AP, 1: 1000, RRID: AB_11232216 ), MYC (Cat#: 60003-2-Ig, 1: 1000, RRID: AB_2734122 ), HA (Cat#: 51064-2-AP, 1: 1000, RRID: AB_11042321 ), CD36 (Cat#: 18836-1-AP, 1:1000, RRID: AB_10597244 ), ASK1 (Cat#: 28201-1-AP, 1: 1000, RRID: AB_2782957 ), MLKL (Cat#: 66675-1-Ig, 1:1000, RRID: AB_2882029 ), and p-ASK1 (Cat#: 28846-1-AP, 1: 1000, RRID: AB_2881220 ) were purchased from Proteintech (Wuhan, China).

    Ubiquitin Proteomics:

    Article Title: USP25 regulates atherosclerosis by restricting RIPK1-mediated inflammatory responses.
    Article Snippet: TNF-α (Cat#: 10291-TA) was purchased from R&D Systems (Minnesota, USA). .. Antibodies against RIPK1 (Cat#: 3493, 1: 1000, RRID: AB_2305314), p-RIPK1 (Cat#: 31122, 1: 1000, RRID: AB_2799000), p-IKKα/β (Cat#: 2697, 1: 1000, RRID: AB_2079382), IKKα (Cat#: 2682, 1:1000, RRID: AB_331626), p-p65 (Cat#: 3033, 1: 1000 for Western bolt and 1: 200 for immunofluorescence, RRID: AB_331284), p65 (Cat#: 8242, 1: 1000, RRID: AB_10859369), p-p38 (Cat#: 4511, 1: 1000, RRID: AB_2139682), p38 (Cat#: 8690, 1: 1000, RRID: AB_10999090), p-JNK (Cat#: 4668S, 1:1000, RRID: AB_ 823588), JNK (Cat#: 9252S, 1:1000, RRID: AB_2250373), p-ERK1/2 (Cat#: 4370S, 1:1000, RRID: AB_2315112), ERK1/2 (Cat#: 4695S, 1:1000, RRID: AB_ 390779), GAPDH (Cat#: 5174, 1: 1000, RRID: AB_ 10622025), α-SMA (Cat#: 19245, 1: 200 for immunofluorescence, RRID: AB_2734735), and Ubiquitin (Cat#: 3936, 1: 1000, RRID: AB_331292) were purchased from Cell Signaling Technology (Danvers, MA, USA). .. Antibodies against FLAG (Cat#: 20543-1-AP, 1: 1000, RRID: AB_11232216), MYC (Cat#: 60003-2-Ig, 1: 1000, RRID: AB_2734122), HA (Cat#: 51064-2-AP, 1: 1000, RRID: AB_11042321), CD36 (Cat#: 18836-1-AP, 1:1000, RRID: AB_10597244), ASK1 (Cat#: 28201-1-AP, 1: 1000, RRID: AB_2782957), MLKL (Cat#: 66675-1-Ig, 1:1000, RRID: AB_2882029), and p-ASK1 (Cat#: 28846- 1-AP, 1: 1000, RRID: AB_2881220) were purchased from Proteintech (Wuhan, China).

    Article Title: USP25 regulates atherosclerosis by restricting RIPK1-mediated inflammatory responses
    Article Snippet: TNF-α (Cat#: 10291-TA) was purchased from R&D Systems (Minnesota, USA). .. Antibodies against RIPK1 (Cat#: 3493, 1: 1000, RRID: AB_2305314 ), p-RIPK1 (Cat#: 31122, 1: 1000, RRID: AB_2799000 ), p-IKKα/β (Cat#: 2697, 1: 1000, RRID: AB_2079382 ), IKKα (Cat#: 2682, 1:1000, RRID: AB_331626 ), p-p65 (Cat#: 3033, 1: 1000 for Western bolt and 1: 200 for immunofluorescence, RRID: AB_331284 ), p65 (Cat#: 8242, 1: 1000, RRID: AB_10859369 ), p-p38 (Cat#: 4511, 1: 1000, RRID: AB_2139682 ), p38 (Cat#: 8690, 1: 1000, RRID: AB_10999090 ), p-JNK (Cat#: 4668S, 1:1000, RRID: AB_823588 ), JNK (Cat#: 9252S, 1:1000, RRID: AB_2250373 ), p-ERK1/2 (Cat#: 4370S, 1:1000, RRID: AB_2315112 ), ERK1/2 (Cat#: 4695S, 1:1000, RRID: AB_390779 ), GAPDH (Cat#: 5174, 1: 1000, RRID: AB_10622025 ), α-SMA (Cat#: 19245, 1: 200 for immunofluorescence, RRID: AB_2734735 ), and Ubiquitin (Cat#: 3936, 1: 1000, RRID: AB_331292 ) were purchased from Cell Signaling Technology (Danvers, MA, USA). .. Antibodies against FLAG (Cat#: 20543-1-AP, 1: 1000, RRID: AB_11232216 ), MYC (Cat#: 60003-2-Ig, 1: 1000, RRID: AB_2734122 ), HA (Cat#: 51064-2-AP, 1: 1000, RRID: AB_11042321 ), CD36 (Cat#: 18836-1-AP, 1:1000, RRID: AB_10597244 ), ASK1 (Cat#: 28201-1-AP, 1: 1000, RRID: AB_2782957 ), MLKL (Cat#: 66675-1-Ig, 1:1000, RRID: AB_2882029 ), and p-ASK1 (Cat#: 28846-1-AP, 1: 1000, RRID: AB_2881220 ) were purchased from Proteintech (Wuhan, China).

    Incubation:

    Article Title: A tumor-resident bacterium promotes cervical cancer progression through lysoPC-mediated c-Jun/c-Fos activation.
    Article Snippet: Equal amounts of protein were separated by SDS-PAGE and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, Billerica, MA, USA). .. Subsequently, the membranes were incubated overnight at 4 ◦ C with primary antibodies against cJun (ab32137; Abcam, London, UK), c-Fos (ab222699; Abcam, London, UK), phospho-c-Jun (S63) (ab32385; Abcam, London, UK), phospho-c-Fos (Ser32) (5348; Cell Signaling Technology, Danvers, MA, USA), MMP2 (ab92536; Abcam, London, UK), MMP9 (ab76003; Abcam, London, UK), JNK (9252; Cell Signaling Technology, Danvers, MA, USA), p-JNK (9251; Cell Signaling Technology, Danvers, MA, USA), ERK (11257-1-AP; Proteintech, China), and p-ERK (28733-1-AP; Proteintech, China) at the indicated doses. .. Following this, the membranes were further incubated with either a goat anti-rabbit IgG secondary antibody (ab6721; Abcam, London, UK) or a goat anti-mouse IgG secondary antibody (ab205719; Abcam, London, UK) for 1 h. To ensure equal loading, an antiGAPDH antibody (ab181602; Abcam, London, UK) was used as a loading control.



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    Effect of Tax on gene expression of <t>the</t> <t>JNK</t> pathway in Cd-induced apoptosis of chicken primary kidney cells. (A) Molecular docking results between Tax and JNK. (B) Cellular Thermal Shift Assay to verify the interaction between Tax and JNK. (C) Relative protein expression of <t>p-JNK/JNK</t> and relative mRNA expression of JNK. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group ( n = 10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.
    P Jnk1 2 3, supplied by Huabio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    p jnk  (Cell Signaling Technology Inc)
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    Knocking down SKA2-induced cell-cycle arrest and apoptosis through the SKA2/ROS/ATM axis in GC cell lines (A) Western blotting analysis of γ-H2AX (Ser139), ATM, p -ATM (Ser1981), p -Chk2 (Thr68), and SKA2 expression in SNU638 and NUGC3 SKA2-knockdown cell lines. (B) Western blotting analysis of SKA2 overexpression in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against γ-H2AX (Ser139), ATM, p -ATM (Ser1981), p -Chk2 (Thr68), and SKA2. (C) Western blotting analysis of KU-55933 treatment in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against cyclin D1, cyclin A2, cyclin B1, p -Chk2 (Thr68), PARP, <t>Cleaved-Caspase3,</t> <t>JNK,</t> p -JNK (Thr183/Tyr185), ATM, p -ATM (Ser1981), and SKA2. (D) Western blotting analysis of BML-277 treatment in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against cyclin D1, cyclin A2, cyclin B1, p -Chk2 (Thr68), and SKA2. (E) Western blotting analysis of P38, p-P38 (Thr180/Tyr182), ERK, p -ERK1/2 (Thr202/Tyr204), JNK, p -JNK (Thr183/Tyr185), and SKA2 expression in SNU638 and NUGC3 SKA2-knockdown cell lines. (F) Western blotting analysis of the rescue effect of SKA2 overexpression on MAPK pathway markers (ERK, p -ERK1/2, JNK, and p -JNK) in SNU638 and NUGC3 SKA2-knockdown cell lines. (G) Western blotting analysis of JNK-IN-8 treatment in SNU638 SKA2-knockdown cell lines using antibodies against PARP, cleaved-caspase3, JNK, p -JNK (Thr183/Tyr185), and SKA2. α-Tubulin was used as the internal control for all blots. Representative blotting images are shown from 3 independent experiments.
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    rabbit anti p jnk  (Bioss)
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    Knocking down SKA2-induced cell-cycle arrest and apoptosis through the SKA2/ROS/ATM axis in GC cell lines (A) Western blotting analysis of γ-H2AX (Ser139), ATM, p -ATM (Ser1981), p -Chk2 (Thr68), and SKA2 expression in SNU638 and NUGC3 SKA2-knockdown cell lines. (B) Western blotting analysis of SKA2 overexpression in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against γ-H2AX (Ser139), ATM, p -ATM (Ser1981), p -Chk2 (Thr68), and SKA2. (C) Western blotting analysis of KU-55933 treatment in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against cyclin D1, cyclin A2, cyclin B1, p -Chk2 (Thr68), PARP, <t>Cleaved-Caspase3,</t> <t>JNK,</t> p -JNK (Thr183/Tyr185), ATM, p -ATM (Ser1981), and SKA2. (D) Western blotting analysis of BML-277 treatment in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against cyclin D1, cyclin A2, cyclin B1, p -Chk2 (Thr68), and SKA2. (E) Western blotting analysis of P38, p-P38 (Thr180/Tyr182), ERK, p -ERK1/2 (Thr202/Tyr204), JNK, p -JNK (Thr183/Tyr185), and SKA2 expression in SNU638 and NUGC3 SKA2-knockdown cell lines. (F) Western blotting analysis of the rescue effect of SKA2 overexpression on MAPK pathway markers (ERK, p -ERK1/2, JNK, and p -JNK) in SNU638 and NUGC3 SKA2-knockdown cell lines. (G) Western blotting analysis of JNK-IN-8 treatment in SNU638 SKA2-knockdown cell lines using antibodies against PARP, cleaved-caspase3, JNK, p -JNK (Thr183/Tyr185), and SKA2. α-Tubulin was used as the internal control for all blots. Representative blotting images are shown from 3 independent experiments.
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    Image Search Results


    Effect of Tax on gene expression of the JNK pathway in Cd-induced apoptosis of chicken primary kidney cells. (A) Molecular docking results between Tax and JNK. (B) Cellular Thermal Shift Assay to verify the interaction between Tax and JNK. (C) Relative protein expression of p-JNK/JNK and relative mRNA expression of JNK. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group ( n = 10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.

    Journal: Poultry Science

    Article Title: Cadmium-induced apoptosis via ROS/JNK pathway in chicken primary kidney cells and antagonism of taxifolin

    doi: 10.1016/j.psj.2026.106920

    Figure Lengend Snippet: Effect of Tax on gene expression of the JNK pathway in Cd-induced apoptosis of chicken primary kidney cells. (A) Molecular docking results between Tax and JNK. (B) Cellular Thermal Shift Assay to verify the interaction between Tax and JNK. (C) Relative protein expression of p-JNK/JNK and relative mRNA expression of JNK. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group ( n = 10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.

    Article Snippet: p-JNK , Wanleibio , 1:500 , WL01813.

    Techniques: Gene Expression, Thermal Shift Assay, Expressing

    The protein levels of cells treated with SP600125 and NAC. (A) Protein expression of p-JNK/JNK, Bad, Bid, Bcl-2, Bax, Bak, DIABLO, XIAP, Caspase-3, and Caspase-9. (B) Quantitative analysis of p-JNK/JNK, Bad, Bid, Bcl-2, Bax, Bak, DIABLO, XIAP, Caspase-3, and Caspase-9 expression. Data are presented as mean ± SD (n = 10). * Indicates a significant difference on Cd + SP600125 or Cd + NAC vs Cd group ( n = 10; * P < 0.05). # Indicates a significant difference between Cd + SP600125 + Tax group and Cd + Tax group ( n = 10; # P < 0.05).

    Journal: Poultry Science

    Article Title: Cadmium-induced apoptosis via ROS/JNK pathway in chicken primary kidney cells and antagonism of taxifolin

    doi: 10.1016/j.psj.2026.106920

    Figure Lengend Snippet: The protein levels of cells treated with SP600125 and NAC. (A) Protein expression of p-JNK/JNK, Bad, Bid, Bcl-2, Bax, Bak, DIABLO, XIAP, Caspase-3, and Caspase-9. (B) Quantitative analysis of p-JNK/JNK, Bad, Bid, Bcl-2, Bax, Bak, DIABLO, XIAP, Caspase-3, and Caspase-9 expression. Data are presented as mean ± SD (n = 10). * Indicates a significant difference on Cd + SP600125 or Cd + NAC vs Cd group ( n = 10; * P < 0.05). # Indicates a significant difference between Cd + SP600125 + Tax group and Cd + Tax group ( n = 10; # P < 0.05).

    Article Snippet: p-JNK , Wanleibio , 1:500 , WL01813.

    Techniques: Expressing

    Knocking down SKA2-induced cell-cycle arrest and apoptosis through the SKA2/ROS/ATM axis in GC cell lines (A) Western blotting analysis of γ-H2AX (Ser139), ATM, p -ATM (Ser1981), p -Chk2 (Thr68), and SKA2 expression in SNU638 and NUGC3 SKA2-knockdown cell lines. (B) Western blotting analysis of SKA2 overexpression in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against γ-H2AX (Ser139), ATM, p -ATM (Ser1981), p -Chk2 (Thr68), and SKA2. (C) Western blotting analysis of KU-55933 treatment in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against cyclin D1, cyclin A2, cyclin B1, p -Chk2 (Thr68), PARP, Cleaved-Caspase3, JNK, p -JNK (Thr183/Tyr185), ATM, p -ATM (Ser1981), and SKA2. (D) Western blotting analysis of BML-277 treatment in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against cyclin D1, cyclin A2, cyclin B1, p -Chk2 (Thr68), and SKA2. (E) Western blotting analysis of P38, p-P38 (Thr180/Tyr182), ERK, p -ERK1/2 (Thr202/Tyr204), JNK, p -JNK (Thr183/Tyr185), and SKA2 expression in SNU638 and NUGC3 SKA2-knockdown cell lines. (F) Western blotting analysis of the rescue effect of SKA2 overexpression on MAPK pathway markers (ERK, p -ERK1/2, JNK, and p -JNK) in SNU638 and NUGC3 SKA2-knockdown cell lines. (G) Western blotting analysis of JNK-IN-8 treatment in SNU638 SKA2-knockdown cell lines using antibodies against PARP, cleaved-caspase3, JNK, p -JNK (Thr183/Tyr185), and SKA2. α-Tubulin was used as the internal control for all blots. Representative blotting images are shown from 3 independent experiments.

    Journal: iScience

    Article Title: SKA2 promotes gastric cancer progression by regulating glutathione metabolism

    doi: 10.1016/j.isci.2026.115202

    Figure Lengend Snippet: Knocking down SKA2-induced cell-cycle arrest and apoptosis through the SKA2/ROS/ATM axis in GC cell lines (A) Western blotting analysis of γ-H2AX (Ser139), ATM, p -ATM (Ser1981), p -Chk2 (Thr68), and SKA2 expression in SNU638 and NUGC3 SKA2-knockdown cell lines. (B) Western blotting analysis of SKA2 overexpression in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against γ-H2AX (Ser139), ATM, p -ATM (Ser1981), p -Chk2 (Thr68), and SKA2. (C) Western blotting analysis of KU-55933 treatment in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against cyclin D1, cyclin A2, cyclin B1, p -Chk2 (Thr68), PARP, Cleaved-Caspase3, JNK, p -JNK (Thr183/Tyr185), ATM, p -ATM (Ser1981), and SKA2. (D) Western blotting analysis of BML-277 treatment in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against cyclin D1, cyclin A2, cyclin B1, p -Chk2 (Thr68), and SKA2. (E) Western blotting analysis of P38, p-P38 (Thr180/Tyr182), ERK, p -ERK1/2 (Thr202/Tyr204), JNK, p -JNK (Thr183/Tyr185), and SKA2 expression in SNU638 and NUGC3 SKA2-knockdown cell lines. (F) Western blotting analysis of the rescue effect of SKA2 overexpression on MAPK pathway markers (ERK, p -ERK1/2, JNK, and p -JNK) in SNU638 and NUGC3 SKA2-knockdown cell lines. (G) Western blotting analysis of JNK-IN-8 treatment in SNU638 SKA2-knockdown cell lines using antibodies against PARP, cleaved-caspase3, JNK, p -JNK (Thr183/Tyr185), and SKA2. α-Tubulin was used as the internal control for all blots. Representative blotting images are shown from 3 independent experiments.

    Article Snippet: p-JNK , Cell Signaling Technology , Cat#9255; RRID: AB_2307321.

    Techniques: Western Blot, Expressing, Knockdown, Over Expression, Control